An atlas of chromatoid body components

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An atlas of chromatoid body components.

The genome of male germ cells is actively transcribed during spermatogenesis to produce phase-specific protein-coding mRNAs and a considerable amount of different noncoding RNAs. Ribonucleoprotein (RNP) granule-mediated RNA regulation provides a powerful means to secure the quality and correct expression of the requisite transcripts. Haploid spermatids are characterized by a unique, unusually l...

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Historical Survey on Chromatoid Body Research

The chromatoid body (CB) is a male reproductive cell-specific organelle that appears in spermatocytes and spermatids. The cytoplasmic granule corresponding to the CB was first discovered some 130 years ago by von Brunn in 1876. Thirty years later the German term "chromatoide Körper" (chromatoid body) was introduced to describe this granule and is still used today. In this review, first, the res...

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PAPOLB/TPAP regulates spermiogenesis independently of chromatoid body-associated factors

Mutant mice lacking a testis-specific cytoplasmic poly(A) polymerase, PAPOLB/TPAP, exhibit spermiogenesis arrest and male infertility. However, the mechanism by which PAPOLB regulates spermiogenesis remains unclear. In this study, we examined the relationships between PAPOLB and other spermiogenesis regulators present in the chromatoid body (CB). The loss of PAPOLB had no impact either on the a...

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The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components.

The chromatoid body is a perinuclear, cytoplasmic cloud-like structure in male germ cells whose function has remained elusive. Here we show that the chromatoid body is related to the RNA-processing body of somatic cells. Dicer and components of microRNP complexes (including Ago proteins and microRNAs) are highly concentrated in chromatoid bodies. Furthermore, we show that Dicer interacts with a...

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The chromatoid body (CB) is a germ granule in the cytoplasm of postmeiotic haploid round spermatids that is loaded with RNA and RNA-binding proteins. Following the discovery of small non-coding RNA-mediated gene regulation and the identification of PIWI-interacting RNAs (piRNAs) that have crucial roles in germ line development, the function of the CB has slowly begun to be revealed. Male germ c...

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ژورنال

عنوان ژورنال: RNA

سال: 2014

ISSN: 1355-8382

DOI: 10.1261/rna.043729.113